Ami Ayalon
- Birth Date
- 6/27/1945
- Birth Place
- Tiberias, British Palestine
- Known For
- Acting

Amihai "Ami" Ayalon (Hebrew: עמיחי "עמי" איילון, born 27 June 1945) is an Israeli politician and a former member of the Knesset for the Labor Party. He was previously head of the Shin Bet, Israel's secret service, and commander-in-chief of the Navy. He came in second to Ehud Barak in a Labor party leadership election in June 2007, and was appointed a Minister without Portfolio in September 2007. He is one of the recipients of Israel's highest decoration, the Medal of Valor.

